Characterization of Ribonucleases from Culture Medium ofLentinus edodes
نویسندگان
چکیده
منابع مشابه
Characterization f Ribonucleases from JSec Culture Medium of Lentinus edocies
g]ycoproteins and their amino acid compesitions are significantly different from that of RNase Le2. In addition to these enzyines, a guanylic acid-specific RNase with a molecular mass 13 kDa was panially purified. Since RNase Le2, which has yery similar IVLterminat sequence to RNase Le 37 and RNase Le 45, was not excreted from the mycelia, the analysis of the structures of these two excreted RN...
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Pancreatic ribonuclease was originally isolated by Kunitz from beef pancreas, and crystallized by the application of classical salt fractionation (1). The protein was subsequently isolated in a highly purified form by Hirs et aE. (2) by column chromatography on IRC-50 (XE-64), a carboxyl type of ion exchange resin, as well as by Martin and Porter (3) who used a partition system on kieselguhr co...
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Lentinula edodes has traditionally been grown on fallen logs. It produces a wide array of enzymes to digest the lignocellulolytic substrate for nutrients. Thus, this organism represents a rich source of potentially potent lignocellulolytic enzymes that can be harnessed for conversion of biomass to simple sugars. These sugars can then be used as feedstock for ethanol production or other chemical...
متن کاملCloning and characterization of the xyn11A gene from Lentinula edodes.
Hemicellulose represents a rich source of biomass that can be converted into useful chemical feedstocks. One of the main components of hemicellulose is xylan, a polymer of xylose residues. Xylanase enzymes that hydrolyze xylan are therefore of great commercial interest. We have cloned a gene (xyn11A) that encodes a 283-amino acid xylanase enzyme from the fungus Lentinula edodes. The enzyme has ...
متن کاملStudies on the Characterization of Ribonucleases.
Ribonucleases with strict hydrolytic specificities for certain internucleotide linkages would be useful for the determination of nucleotide sequences in a polynucleotide chain since they would produce enzymatic digests of ribonucleic acid containing oligonucleotides of partially definedcomposition. At the present time, two types of endonuclease specificity are known: the pyrimidine specificity ...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1998
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.62.1604